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KMID : 0545120100200101424
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 10 p.1424 ~ p.1429
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Synthesis of an amphiphilic poly(γ-glutamic acid)-cholesterol conjugate and its application as an artificial chaperone
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Lee Eun-Hye
Yoshiki Kamigaito
Takashi Tsujimoto
Hiroshi Uyama
Sung Moon-Hee
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Abstract
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A poly(γ-glutamic acid) (γPGA)-cholesterol conjugate was synthesized and the properties of an aqueous solution were evaluated. The conjugate showed amphiphilic nature derived from the hydrophilic γPGA backbone and the hydrophobic cholesterol side chain. The conjugate spontaneously formed nanoparticles in the aqueous solution of the low concentration, and the high concentration resulted in the formation of the physical gel. By utilizing the self-aggregating properties of the conjugate in water, an artificial chaperone was developed. The complex of protein with the nanoparticles of the conjugate was formed and the protein was released upon the dissociation of the nanoparticles by the addition of β-cyclodextrin. For denatured carbonic anhydrase, the activity was recovered in the artificial chaperone of the nanoparticle conjugate.
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KEYWORD
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Poly(γ-glutamic acid), cholesterol, amphiphilic polymer, artificial chaperone
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